| Autor: |
Shang, Yutong, Wang, Yan, Zhang, Ni, Liu, Fei, Ding, Mingzhu, Yuan, Yingjin |
| Zdroj: |
Journal of Environmental Chemical Engineering; August 2024, Vol. 12 Issue: 4 |
| Abstrakt: |
Bioremediation is a promising technology to treat the petroleum hydrocarbon contamination. In this study, the strengthen of alkane metabolism and transport activity in Yarrowia lipolyticaresulted in increased degradation of n-hexadecane. The highest degradation rate of 10 g/L n-hexadecane of engineered Y. lipolyticawas 75.12 % at 96 h, and the wild-type was 45 %. Candida bombicola,which could produce sophorolipids, was cultured with the engineered Y. lipolyticato construct microbial consortia for improving the degradation. The highest degradation rate by the microbial consortia reached 81.57 % at 96 h. Transcriptomic analysis showed the expression levels of genes related to alkane metabolism in engineered Y. lipolyticawas down-regulated. Thus, the alcohol dehydrogenase activity was further strengthened, and the degradation rate of 20 g/L n-hexadecane were significantly increased. The 20 g/L n-hexadecane degradation rate of newly engineered Y. lipolyticareached 57.89 %, and that of the microbial consortium reached 72.28 %. This study provides new insights into the construction of synthetic microbial consortia for high-efficient degradation of n-hexadecane. |
| Databáze: |
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