Functional expression of a human C5a receptor clone in Xenopusoocytes requires additional RNA

Autor: Kroll, Beate, Emde, Monica, Jeromin, Andreas, Penner, Lothar, Rechkemmer, Gerhard, Kretzschmar, Titus, Klos, Andreas, Köhl, Jörg, Bautsch, Wilfried
Zdroj: FEBS Letters; January 1991, Vol. 291 Issue: 2 p208-210, 3p
Abstrakt: cRNA from a PCR-generated C5aR clone was prepared by in vitro transcription and microinjected into Xenopus laevisoocytes. Ligand-induced whole cell current could be detected after co-injection of cRNA for the C5aR with total RNA of the unstimulated U937 cell line, but not with either of the components injected alone. These data clearly demonstrate an absolute requirement of the C5aR for an additional human factor to become functionally expressed in Xenopusoocytes.
Databáze: Supplemental Index