Crystallization of the photosynthetic light-harvesting pigment-protein complex B800-850 of Rhodopseudomonas capsulata

Autor: Welte, Wolfram, Wacker, Thomas, Leis, Monika, Kreutz, Werner, Shiozawa, Judith, Gad'on, Nasser, Drews, Gerhart
Zdroj: FEBS Letters; January 1985, Vol. 182 Issue: 2 p260-264, 5p
Abstrakt: The B800-850 light-harvesting complex of Rhodopseudomonas capsulatawas crystallized in the presence of detergents. The crystals were obtained by a vapour diffusion technique, using polyethylene glycol as a precipitant. Crystals grew to a size of 0.5 × 0.5 × 0.3 mm within two weeks. Two different crystal forms were obtained; one is supposed to be triclinic, space group PI, the other orthorhombic, space group C222 1. Both crystal forms diffract to approximately 1.0 nm. Absorption spectra and polyacrylamide gel electrophoresis demonstrate that all expected components, i.e. three polypeptides of apparent Mr,8000, 10000 and 14000, bacteriochlorophyll aand carotenoids, are present and in native conformation.
Databáze: Supplemental Index