Carbohydrates in mammalian tryptophanyl-tRNA synthetase

Autor: Kovaleva, Galina K., Zheltova, Anna O., Nikitushkina, Tatyana V., Egorov, Tsezi A., Musoljamov, Aleksander Ch., Kisselev, Lev L.
Zdroj: FEBS Letters; January 1992, Vol. 309 Issue: 3 p337-339, 3p
Abstrakt: Homogeneous preparations of bovine tryptophanyl-tRNA synthetase (EC 6.1.1.2) contain monosaccharides (mannose, fucose, galactose, N-acetylglucosamine) as revealed by liquid chromatography. Their content comprises 2.5–3.0% (w/w) of the enzyme composed of two subunits (60 kDa × 2). The same set of sugars was detected in clastase and CNBr-generated fragments (with molecular masses of approx. 40 kDa and 30 kDa, respectively). It is concluded that bovine tryptophanyl-tRNA synthetase, in addition to being a metallo- and phosphoprotein, is also a glycoprotein.
Databáze: Supplemental Index