| Autor: |
You, Shijun, Yao, Shuyuan, Chen, Xuanhao, Hou, Qing, Liu, Zhaoxia, Lei, Gaoke, Xie, Xuefeng, Liang, Zhengjiao, Yuchi, Zhiguang, You, Minsheng, Liu, Yuanyuan, Xiong, Lei |
| Zdroj: |
Journal of Agricultural and Food Chemistry; April 2024, Vol. 72 Issue: 14 p8180-8188, 9p |
| Abstrakt: |
Juvenile hormone binding protein (JHBP) is a key regulator of JH signaling, and crosstalk between JH and 20-hydroxyecdysone (20E) can activate and fine-tune the mitogen-activated protein kinase cascade, leading to resistance to insecticidal proteins from Bacillis thuringiensis(Bt). However, the involvement of JHBP in the Bt Cry1Ac resistance of Plutella xylostellaremains unclear. Here, we cloned a full-length cDNA encoding JHBP, and quantitative real-time PCR (qPCR) analysis showed that the expression of the PxJHBPgene in the midgut of the Cry1Ac-susceptible strain was significantly higher than that of the Cry1Ac-resistant strain. Furthermore, CRISPR/Cas9-mediated knockout of the PxJHBPgene significantly increased Cry1Ac susceptibility, resulting in a significantly shorter lifespan and reduced fertility. These results demonstrate that PxJHBPplays a critical role in the resistance to Cry1Ac protoxin and in the regulation of physiological metabolic processes associated with reproduction in adult females, providing valuable insights to improve management strategies of P. xylostella. |
| Databáze: |
Supplemental Index |
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