Prevention of Post-operative Bacterial Colonization on Mice Buccal Mucosa Using Biogenic Silver Nanoparticles-Coated Nylon Sutures

Autor: Syukri, Dwi Marlina, Singh, Sudarshan, Nwabor, Ozioma Forstinus, Ontong, Julalak Chorachoo, Dejyong, Krittee, Sunghan, Jutapoln, Dejyong, Kwanchanok, Lethongkam, Sakkarin, Voravuthikunchai, Supayang Piyawan
Zdroj: Regenerative Engineering and Translational Medicine; 20240101, Issue: Preprints p1-15, 15p
Abstrakt: Purpose: In this study, biogenic silver nanoparticles (AgNPs) deposited on nylon sutures using in situtechnique were employed as an in vivoantibacterial and wound healing strategy on post-operative buccal mucosa. Methods: Biogenic AgNPs synthesized using phenolic-rich Eucalyptus camaldulensisextract, followed by coating on nylon monofilament surgical sutures. Subsequently, coated nylon sutures were sewn with a simple interrupt pattern at the wound of the buccal mucosa of five mice per group (coated and uncoated). Sutures were taken out at days 1, 3, and 7 post-surgery, and a biopsy specimen of buccal mucosa tissues was removed. In vivoeffects including antibacterial activity, nitric oxide test, and histopathological changes were demonstrated in the buccal cavity of the mice model. Results: AgNPs-coated nylon sutures revealed bacteriostatic effects with more than 90% reduction in bacterial growth. Coated sutures inhibited nitric oxide generation by 12.09 μM, compared with uncoated sutures. In addition, the hematoxylin and eosin staining of buccal mucosa demonstrated that AgNPs-coated sutures attenuated inflammatory cells at wound sites. The average number of fibroblast cells in the AgNPs-coated suture group increased up to 0.24 cell/mm2, compared with the uncoated group (p> 0.05). Conclusions: This pioneering work suggested a reliable strategy for the management of surgical sites that promotes the wound healing due to the antibacterial and anti-inflammatory properties of AgNPs-coated nylon sutures. Lay Summary: Phenolic-rich Eucalyptus camaldulensisaqueous leaf extract was used to synthesize AgNPs. The development of biogenic AgNPs-coated nylon sutures using in situtechnique represented a recent effort aimed at reducing postoperative surgical site infections by preventing bacterial contamination and inflammation.
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