| Autor: |
Wang, Xingdong, Ge, Yizhi, Hou, Yuting, Wang, Xiaodong, Yan, Zhengcun, Li, Yuping, Dong, Lun, She, Lei, Tang, Can, Wei, Min, Zhang, Hengzhu |
| Zdroj: |
Cancer Gene Therapy; 20240101, Issue: Preprints p1-12, 12p |
| Abstrakt: |
Patients diagnosed with glioblastoma (GBM) have the most aggressive tumor progression and lethal recurrence. Research on the immune microenvironment landscape of tumor and cerebrospinal fluid (CSF) is limited. At the single-cell level, we aim to reveal the recurrent immune microenvironment of GBM and the potential CSF biomarkers and compare tumor locations. We collected four clinical samples from two patients: malignant samples from one recurrent GBM patient and non-malignant samples from a patient with brain tumor. We performed single-cell RNA sequencing (scRNA-seq) to reveal the immune landscape of recurrent GBM and CSF. T cells were enriched in the malignant tumors, while Treg cells were predominately found in malignant CSF, which indicated an inhibitory microenvironment in recurrent GBM. Moreover, macrophages and neutrophils were significantly enriched in malignant CSF. This indicates that they an important role in GBM progression. S100A9, extensively expressed in malignant CSF, is a promising biomarker for GBM diagnosis and recurrence. Our study reveals GBM’s recurrent immune microenvironment after chemoradiotherapy and compares malignant and non-malignant CSF samples. We provide novel targets and confirm the promise of liquid CSF biopsy for patients with GBM. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full text from SpringerLink