| Autor: |
Mei, Yuan, Hai, Zijuan, Li, Ziyun, Rong, Kuanrong, Tang, Wenjian, Song, Qin-Hua |
| Zdroj: |
Analytical Chemistry; 20240101, Issue: Preprints |
| Abstrakt: |
Fluoride anions (F–) play a crucial role in human physiological processes. However, excessive intake of F–would affect oxygen metabolism and promote the generation of oxygen-free radicals. Hence, it is essential to develop a precise and efficient fluorescent probe for visualizing F–-induced oxidative stress. In this work, we developed the first bifunctional BODIPY-based fluorescent probe dfBDP with p-tert-butyldimethylsilanolate benzyl thioether as the sensing site for the detection of F–and HClO via two distinct reactions, the self-immolative removal and the thioether oxidation, which generate the sensing products with two nonoverlap fluorescence bands: 800–1200 and 500–750 nm, respectively. The probe dfBDP displays rapid response, high specificity, and sensitivity for the detection of F–(LOD, 316.2 nM) and HClO (LOD, 33.9 nM) in vitro. Cellular imaging reveals a correlation between F–-induced oxidative stress and the upregulation of HClO. Finally, probe dfBDP was employed to detect F–and HClO in mice under the stimulation of F–. The experimental results display that the level of HClO elevates in the liver of mice. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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