| Autor: |
Mosbæk, Freya, Kjeldal, Henrik, Mulat, Daniel G, Albertsen, Mads, Ward, Alastair J, Feilberg, Anders, Nielsen, Jeppe L |
| Zdroj: |
The ISME Journal; October 2016, Vol. 10 Issue: 10 p2405-2418, 14p |
| Abstrakt: |
Inhibition of anaerobic digestion through accumulation of volatile fatty acids occasionally occurs as the result of unbalanced growth between acidogenic bacteria and methanogens. A fast recovery is a prerequisite for establishing an economical production of biogas. However, very little is known about the microorganisms facilitating this recovery. In this study, we investigated the organisms involved by a novel approach of mapping protein-stable isotope probing (protein-SIP) onto a binned metagenome. Under simulation of acetate accumulation conditions, formations of 13C-labeled CO2and CH4were detected immediately following incubation with [U-13C]acetate, indicating high turnover rate of acetate. The identified 13C-labeled peptides were mapped onto a binned metagenome for improved identification of the organisms involved. The results revealed that Methanosarcinaand Methanoculleuswere actively involved in acetate turnover, as were five subspecies of Clostridia.The acetate-consuming organisms affiliating with Clostridiaall contained the FTFHS gene for formyltetrahydrofolate synthetase, a key enzyme for reductive acetogenesis, indicating that these organisms are possible syntrophic acetate-oxidizing (SAO) bacteria that can facilitate acetate consumption via SAO, coupled with hydrogenotrophic methanogenesis (SAO-HM). This study represents the first study applying protein-SIP for analysis of complex biogas samples, a promising method for identifying key microorganisms utilizing specific pathways. |
| Databáze: |
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