| Autor: |
Sugihara, Yuta, Nakata, Yuki, Yamayoshi, Asako, Murakami, Akira, Kobori, Akio |
| Zdroj: |
Chemistry Letters; August 2017, Vol. 46 Issue: 8 p1265-1268, 4p |
| Abstrakt: |
We synthesized phosphorothioated 2′-OMe RNAs containing PXA residues at the 5′-end of the strands (PXA-ORNs) for sequence-selective inhibition of gene expression of GFP in HeLa cells stably expressing GFP. The inhibitory efficiency is evaluated by measuring the fluorescent intensity of the expressed GFP. Cell-based assay revealed that under UV irradiation, PXA-ORNs greatly inhibit the gene expression of GFP (by as much as 60%).We synthesized phosphorothioated 2′-OMe RNAs containing PXA residues at the 5′-end of the strands (PXA-ORNs) for sequence-selective inhibition of gene expression of GFP in HeLa cells stably expressing GFP. The inhibitory efficiency is evaluated by measuring the fluorescent intensity of the expressed GFP. Cell-based assay revealed that under UV irradiation, PXA-ORNs greatly inhibits the gene expression of GFP (by as much as 60%). |
| Databáze: |
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