| Abstrakt: |
Charcoal rot causes severe yield loss in mungbean. To sustain the crop health, it is vital to include alleles that may be useful in resisting the charcoal rot disease. Therefore, in the present study, genetic divergence using ISSR primers across six samples were studied for charcoal rot resistance. One resistant and one susceptible cultivars of mung bean namely, WBM 4–34-1–1 and PDM-139, respectively were used in the present investigtion. Leaf samples were treated with an elicitor, Salicyclic acid (SA) at 1 milli molar (mM) and Macrophomina phaseolinawas inoculated at 15 DAS and untreated seedlings served as a check. Total genomic DNA was isolated from leaf samples of 15 days old seedlings of both the genotypes and ISSR genotyping was performed with six ISSR primers i.e., UBC- 847, ISSCR 2, ISSCR 5, UBC 825, A9 and C6. The primers UBC-847 and ISSCR-2 were observed to show moderate polymorphism of 15.15%. The UPGMA clustering method differentiated the test samples into three clusters i.e., Cluster I includes only one sample viz., Bireshwar genotype treated with Salicyclic acid @ 1 mM. Cluster II was the major one that included three samples viz.., Bireshwar genotype (Control), Samrat genotype (Control) and Samrat genotype of mungbean treated with Salicyclic acid @ 1 mM (SA @1 mM) and Cluster III included two samples viz. Bireshwar treated with salicyclic acid @ 1 mM and inoculated with pathogen (SA@1 mM with Pathogen Inoculated), Samrat genotype treated with salicyclic acid @ 1 mM and inoculated with pathogen (SA@1 mM with Pathogen Inoculated). These findings indicate that the mungbean genotypes used in the present study could enrich the local gene pool and provide information for charcoal rot resistance breeding. |
Full text from SpringerLink