| Autor: |
Low, Teresa L.K., McClure, John E., Naylor, Paul H., Spangelo, Bryan L., Goldstein, Allan L. |
| Zdroj: |
Journal of Chromatography A; August 1983, Vol. 266 Issue: 1 p533-544, 12p |
| Abstrakt: |
High-performance liquid chromatography (μBondapak C18column with 0.05 % trifluoroacetic acid in acetonitrile as solvent system) was used to isolate thymosin α1, (α1) from thymosin fraction 5 (f5) of various species (calf, pig, sheep and mouse). Each of the f5 preparations gave a protein peak similar in retention time to bovine thymosin α1. This peak coincided with the immunoreactive peak determined by a radioimmunoassay for α1. Chromatographic analysis of fresh thymus tissue extracts using a high-performance liquid chromatographic similar system did not reveal a detectable protein peak or immunoreactive peak at the α1position. Our results suggest that α1may be synthesized in a precursor form in animal tissues. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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