| Abstrakt: |
The soil-borne polyphagous pathogen Macrophomina phaseolinais highly damaging and has expanded its range and diversity. Ten sesame root rot pathogenic M. phaseolinaisolates were collected from various parts of Odisha, India and subjected to molecular analysis and phenotypic characterization. In molecular assays, 27 bands representing 71.9% polymorphism were obtained for genetic diversity with four ISSR primers. Among them, ISSR9 had the highest PIC value of 0.663. The isolates were divided into three groups, each sharing 60% of their genetic makeup, according to UPGMA clustering method. The dendrogram and principal component analysis (PCA) ordination of isolates revealed a reliable correlation between geographic origin and the pathogen’s genetic foundation. The isolates showed comparable clustering based on phenotypic characteristics and pathogenicity. There was a wide range of phenotypic (colony colour, colony type, growth rate, and the size, shape, colour, and abundance of the aerial mycelium microsclerotia) and virulence among the various isolates (against sesame cultivar VRI-1). Microsclerotia size was found to be negatively correlated to virulence. The results corroborate the existence of a robust association between phenotypic traits, virulence, and the genetic foundation of the pathogen, and furthermore, confirm the occurrence of heterogeneity among the isolates. The pathogen population structure may be better grasped with this kind of information on its defining characteristics. |
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