| Abstrakt: |
Purpose: Cariprazine hydrochloride (CPZ) is a new atypical antipsychotic. A nanostructured lipid carrier (NLC) loaded with cariprazine hydrochloride was prepared by the emulsification solvent evaporation method. The HPTLC technique for cariprazine hydrochloride has not yet been established. Methods: A simple and rapid High-Performance Thin-Layer Chromatography (HP-TLC) was developed and validated for the quantification of cariprazine hydrochloride-loaded nanostructured lipid carrier on silica gel 60F254plates, using toluene: methanol (7:3 V/V) as mobile phase. The analysis was performed at 253 nm. The method was validated according to the International Council for Harmonisation guidelines with respect to linearity, range, precision, accuracy, specificity, and robustness. Results: Cariprazine hydrochloride presents as a sharp band at Rf0.64, with a good linear relationship with a concentration range of 1–5 µL/band with a regression coefficient of 0.993. The limit of detection and limit of quantification was found to be 0.50 μg/band and 1.52 µg/band respectively. The method was found to be precise and reliable. Conclusion: The technique is appropriate for the routine examination of cariprazine in dosage forms and in bulk. The analysis method was found to be simple, accurate, and economical. |
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