| Autor: |
Sakaguchi, Shunta, Mizuno, Sonoko, Okochi, Yasushi, Tanegashima, Chiharu, Nishimura, Osamu, Uemura, Tadashi, Kadota, Mitsutaka, Naoki, Honda, Kondo, Takefumi |
| Zdroj: |
Cell Reports; 20230101, Issue: Preprints |
| Abstrakt: |
During development, positional information directs cells to specific fates, leading them to differentiate with their own transcriptomes and express specific behaviors and functions. However, the mechanisms underlying these processes in a genome-wide view remain ambiguous, partly because the single-cell transcriptomic data of early developing embryos containing accurate spatial and lineage information is still lacking. Here, we report a single-cell transcriptome atlas ofDrosophilagastrulae, divided into 77 transcriptomically distinct clusters. We find that the expression profiles of plasma-membrane-related genes, but not those of transcription factor genes, represent each germ layer, supporting the nonequivalent contribution of each transcription factor mRNA level to effector gene expression profiles at the transcriptome level. We also reconstruct the spatial expression patterns of all genes at the single-cell stripe level as the smallest unit. This atlas is an important resource for the genome-wide understanding of the mechanisms by which genes cooperatively orchestrate Drosophilagastrulation. |
| Databáze: |
Supplemental Index |
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