| Autor: |
Angal, Ashvini, Shidture, Shubham, Syed, Jaserah, Tiwari, Deepika Pandey, Dubey, Ashok Kumar, Bhaduri, Anirban, Pujari, Radha |
| Zdroj: |
International Microbiology; 20230101, Issue: Preprints p1-12, 12p |
| Abstrakt: |
In this study, seven strains of Limosilactobacillus fermentumwere isolated from an infant fecal sample and characterized using in vitro studies. Lactobacillus rhamnosusGG was used as a comparison because it is a well-documented commercial probiotic. The isolates were tested for attributes such as acid and phenol tolerance, bile salt hydrolase (BSH) activity, and antibiotic sensitivity. One isolate, L. fermentumFS-10,displayed enhanced cell surface hydrophobicity (> 85%) and mucin adhesion. Mucin-binding helps colonization in the gut. The immunomodulatory property of L. fermentumFS-10 was evaluated by determining the modulation of pro- and anti-inflammatory factors such as tumor necrosis factor-alpha (TNF-α), interleukin (IL)-10, and nitric oxide (NO) in human acute monocytic leukemia (THP-1) cells under inflammatory conditions induced by lipopolysaccharide (LPS). L. fermentumFS-10 potently downregulated the expression of TNF-α and nitric oxide and upregulated IL-10 levels, indicating an anti-inflammatory response. Safety assessment of the strain revealed the absence of genes for virulence factors, toxin production, and antibiotic resistance, potentiating application as a probiotic strain. |
| Databáze: |
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