| Autor: |
Amin, Shrunali, Basu, Malika, Buzinova, Valeria, Delgado, Anthony, Mahadevan, Tejas, Mishra, Sanya, Zaida, Sarah, Wang, Xi, Sokac, Anna Marie |
| Zdroj: |
STAR Protocols; September 2023, Vol. 4 Issue: 3 |
| Abstrakt: |
The dialdehyde glyoxal is an alternative chemical fixative that cross-links tissues faster than formaldehyde, retains higher antigenicity, and is less hazardous than either formaldehyde or glutaraldehyde. Here we present a glyoxal-based fixation protocol for use with Drosophilaembryos. We describe steps to prepare acid-free glyoxal, fix embryos, and then stain with antibodies for immunofluorescence (IF). We also describe methods for RNA fluorescence in situhybridization (FISH) and FISH plus IF (FISH-IF) using glyoxal-fixed embryos. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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