| Autor: |
ZHANG, Bo, FAN, Wen-min, ZHU, Zhen-zhen, WANG, Ying, ZHAO, Zheng-yang |
| Zdroj: |
Journal of Integrative Agriculture; March 2023, Vol. 22 Issue: 3 p762-775, 14p |
| Abstrakt: |
Sugar content is a determinant of apple (Malus×domesticaBorkh.) sweetness. However, the molecular mechanism underlying sucrose accumulation in apple fruit remains elusive. Herein, this study reported the role of the sucrose transporter MdSUT2.1 in the regulation of sucrose accumulation in apples. The MdSUT2.1gene encoded a protein with 612 amino acid residues that could be localized at the plasma membrane when expressed in tobacco leaf protoplasts. MdSUT2.1was highly expressed in fruit and was positively correlated with sucrose accumulation during apple fruit development. Moreover, complementary growth assays in a yeast mutant validated the sucrose transport activity of MdSUT2.1. MdSUT2.1overexpression in apples and tomatoes resulted in significant increases in sucrose, fructose, and glucose contents compared to the wild type (WT). Further analysis revealed that the expression levels of sugar metabolism- and transport-related genes SUSYs, NINVs, FRKs, HXKs, and TSTsincreased in apples and tomatoes with MdSUT2.1overexpression compared to WT. Finally, unlike the tonoplast sugar transporters MdTST1 and MdTST2, the promoter of MdSUT2.1was not induced by exogenous sugars. These findings provide valuable insights into the molecular mechanism underlying sugar accumulation in apples. |
| Databáze: |
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