| Autor: |
Banga, Satnam S., Hall, Kathryn T., Sandhu, Arbansjit K., Weaver, David T., Athwal, Raghbir S. |
| Zdroj: |
Mutation Research/DNA Repair; November 1994, Vol. 315 Issue: 3 p239-247, 9p |
| Abstrakt: |
Cells derived from mice homozygous for the severe combined immune deficiency (scid) mutation exhibit hypersensitivity to ionizing radiation, and defects in DNA double-strand break repair and V(D)J recombination. Using the technique of microcell-mediated chromosome transfer, we have introduced a number of dominantly marked human chromosomes into scidcells to localize the human homolog of the murine scidgene. Analysis of human-scidhybrid clones revealed that the presence of human chromosome 8 partially restored accurate V(D)J recombination and radioresistance to scidcells. Subsequent loss of the human chromosome 8 from human-scidhybrid clones rendered these cells sensitive to γ-radiation and impaired their ability to catalyse V(D)J recombination. Introduction of chromosomes 2, 14, 16 and 19 that encode other repair genes did not result in the correction of these two sciddefects. These observations demonstrate that the human homolog of the mouse scidgene resides on human chromosome 8. |
| Databáze: |
Supplemental Index |
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