| Autor: |
Kitajima, Chihiro, Ichijo, Toshihiro, Ichikawa-Seki, Madoka, Kitajima, Chihiro, Ichijo, Toshihiro, Ichikawa-Seki, Madoka |
| Zdroj: |
Parasite - Journal de la Société Française de Parasitologie; January 2022, Vol. 29 Issue: 1 |
| Abstrakt: |
Setaria marshalliis a mosquito-borne filarial nematode that causes infection in calves younger than two years old. In the present study, nematodes were obtained from a calf in Japan and morphologically identified as S. marshalli. Additionally, the partial cytochrome oxidase subunit I (COI) region (596 bp) was analyzed for the first time to establish a reliable DNA barcode. Nucleotide sequences of COIwere identical among the seven worms obtained. The COIregion can be a useful marker for species discrimination in the case of S. marshallisince nucleotide variations observed between the closest congener, Setaria cervi(51/596 bp), were sufficient to allow species discrimination. However, the phylogenetic relationship of S. marshalliwith its congeners was unclear in a maximum likelihood tree. We found that the partial COIsequence of S. marshallianalyzed in the present study matched a relevant section of the complete mitochondrial genome of S. labiatopapillosathat was deposited in the International Nucleotide Sequence Database. This finding suggests that S. marshalliwas misdiagnosed as S. labiatopapillosain a previous study. It is crucial to conduct accurate morphological analyses to obtain reliable molecular information regarding Setarianematodes. |
| Databáze: |
Supplemental Index |
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