| Autor: |
Alfarafisa, Nayla Majeda, Kitaguchi, Kohji, Yabe, Tomio |
| Zdroj: |
Bioscience, Biotechnology, and Biochemistry; February 2021, Vol. 85 Issue: 2 p430-439, 10p |
| Abstrakt: |
Under oxidative stress, reactive oxygen species (ROS) alter signal transduction and induce macromolecular damage in cells. Such oxidative damage can lead to sarcopenia, an age-related syndrome characterized by a progressive loss of mass and strength of skeletal muscles. Because food components do not directly come in contact with muscle cells, we focused on the effects of secretions produced by stimulated intestinal epithelial cells on oxidative stress in myoblast cells. An extract of Diospyros kakiwas fractionated using different concentrations of ethanol. Each fraction showed different levels of antioxidant and phenolic compounds. The biological activity was evaluated using a Caco-2 cell coculture system. Secretions from Caco-2 cells exposed to 0.5 mg/mL D. kakiextract attenuated the oxidative stress-induced reduction of C2C12 cell viability, suggesting that the D. kakiextract could stimulate intestinal epithelial cells to produce secretions that reduce oxidative stress in myoblasts in vitro.Graphical AbstractThe protective effect of persimmon extracts on C2C12 cells subjected to oxidative stress was measured using components secreted from differentiated Caco-2 cells. |
| Databáze: |
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