Isolation of a Novel Alphabaculovirus(Baculoviridae) from Automeris liberia(Cramer, 1780) (Lepidoptera: Saturniidae) in African Oil Palms in Brazil

Autor: Guzzo, Elio Cesar, Silva, Kelly Barbosa, Silva, Chryslane Barbosa, Chia, Gilson Sanchez, Tinôco, Ricardo Salles, de Souza, Marlinda Lobo, Sanches, Marcio Martinello, Acevedo, Juan Pablo Molina, Goulart, Henrique Fonseca, Santana, Antonio Euzébio Goulart
Zdroj: Neotropical Entomology; August 2022, Vol. 51 Issue: 4 p637-640, 4p
Abstrakt: A novel baculovirus observed to infect Automeris liberia (Cramer) (bullseye moth) is here described. Caterpillars of A. liberiawith symptoms of viral infection were collected from African oil palm plantations in Tailândia, PA, Brazil. Macerated caterpillars were then offered to caterpillars of Automeris cinctistriga (Felder & Rogenhoper), leading to viral symptoms and death before pupation. A transmission electron microscope was used for virus ultrastructural identification. The presence of viral occlusion bodies (OBs) containing multiple nucleocapsids was observed and such features are compatible with Alphabaculovirus(Baculoviridae). Molecular detection by PCR with primers for polyhedrin gene (polh) and for late expression factor-8 gene (lef-8), confirmed that this isolate belonged to Alphabaculovirusgenus. To our knowledge, this is the first record of a baculovirus isolated from or associated to Automeris. The name Automeris liberia nucleopolyhedrovirus(AuliNPV) is proposed for the new virus.
Databáze: Supplemental Index