Interaction of Corticosterone Antibody and Glutaraldehyde Adsorbed on Pt Electrodes Modified with Silane

Autor: Kim, Il Kwang, Park, Young Ok, Chun, Hyun Ja
Zdroj: Analytical Sciences; April 1996, Vol. 12 Issue: 2 p327-330, 4p
Abstrakt: The interaction of a corticosterone antibody and glutaraldehyde adsorbed on a Pt electrode modified with silane was examined by cyclic voltammetry. A mirror-polished Pt wire was oxidized for 10 min at +1.3 V vs.Ag/AgCl in 1,0 M H2SO4. The oxidized Pt wire was coated with 3-aminopropyltrimethoxysilane under N2gas for 1 h at room temperature in benzene, and then modified with glutaraldehyde. The modified electrodes were tested for their electrocatalytic properties and interaction with the corticosterone antibody. The cathodic peak currents (-0.4 V) exhibited a reproducible linearity of the current intensity vs.the concentration of anticorticosterone.
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