| Abstrakt: |
The demand for herbal raw material has increased and there is a requirement for quality control to look at the integrity of the herbal raw materials. There is a need for an easy and fast technique to check the quality and the authenticity of these raw materials. Nelumbo nuciferaseeds have been used as traditional medicine for ages for curing various diseases. Its microscopy examination of fruit showed double-layered palisade tissue, rosette scleride, and epidermal pores. The morphology of starch grains were found to be simple, oval, and elliptical to polygonal in shape, which is the unique characteristic of the plant. Phytochemical analysis showed the presence of alkaloids, flavonoids, tannins, phenols, and saponin. A comparative study of cold and hot extraction of seed extract was carried out with respect to the HPTLC fingerprint profile. The HPTLC fingerprint of the cold and hot extract was done using butanol: water: acetic acid (4:1:1v/v/v) it showed the presence of 9 bands (RF0.05–0.94) on the plate. The result shows that both cold and hot extract can be used for fingerprinting. Different concentrations of cold extract (100%, 80%, 50%, 20%) was used to standardize the lowest on plate concentration which can give maximum bands with high resolution, the results shows that a lower concentration of 50% can be used for the study. Thus, microscopy and HPTLC fingerprinting data along with morphological information can be used as a quality control tool to check the authenticity of the Nelumbo nuciferawhich has potential therapeutic value. |
Full text from SpringerLink