| Autor: |
Sturme, Mark H. J., van der Berg, Jan Pieter, Bouwman, Lianne M. S., De Schrijver, Adinda, de Maagd, Ruud A., Kleter, Gijs A., Battaglia-de Wilde, Evy |
| Zdroj: |
ACS Agricultural Science & Technology; April 2022, Vol. 2 Issue: 2 p192-201, 10p |
| Abstrakt: |
CRISPR-Cas-based genome editing allows for precise and targeted genetic modification of plants. Nevertheless, unintended off-target edits can arise that might confer risks when present in gene-edited food crops. Through an extensive literature review we gathered information on CRISPR-Cas off-target edits in plants. Most observed off-target changes were small insertions or deletions (1–22 bp) or nucleotide substitutions, and large deletions (>100 bp) were rare. One study detected the insertion of vector-derived DNA sequences, which is important considering the risk assessment of gene-edited plants. Off-target sites had few mismatches (1–3 nt) with the target sequence and were mainly located in protein-coding regions, often in target gene homologues. Off-targets edits were predominantly detected via biased analysis of predicted off-target sites instead of unbiased genome-wide analysis. CRISPR-Cas-edited plants showed lower off-target mutation frequencies than conventionally bred plants. This Review can aid discussions on the relevance of evaluating off-target modifications for risk assessment of CRISPR-Cas-edited plants. |
| Databáze: |
Supplemental Index |
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