| Autor: |
Varca, Anthony C., Casalena, Dominick, Auld, Douglas, Buhrlage, Sara J. |
| Zdroj: |
STAR Protocols; December 2021, Vol. 2 Issue: 4 |
| Abstrakt: |
Identification of selective deubiquitinase (DUB) inhibitors is critical for probe development to further understand and explore DUB biological function. Here, we detail the optimization and deployment of an in vitrofluorogenic ubiquitin-rhodamine assay to conduct high-throughput screening of a small molecule library against a panel of DUBs. In screening the compound library against multiple DUBs in parallel, we describe an approach for identifying selective DUB inhibitors and provide a roadmap for enabling selective DUB inhibitor discovery. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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