Safe Mycobacterium tuberculosisDNA Extraction Method That Does Not Compromise Integrity

Autor: Warren, Robin, de Kock, Marianna, Engelke, Erica, Myburgh, Roseline, Gey van Pittius, Nico, Victor, Thomas, van Helden, Paul
Zdroj: Journal of Clinical Microbiology; January 2006, Vol. 44 Issue: 1 p254-256, 3p
Abstrakt: ABSTRACTConcern has been raised about the efficacy of the heat killing of mycobacteria during the isolation of DNA. We demonstrate a method that allows for the efficient killing of Mycobacterium tuberculosiswithout compromising DNA integrity for subsequent molecular investigation. This method reduces the risk of infection to the research scientist.
Databáze: Supplemental Index