Constitutive and inducible kappa B binding activities in the cytosol of v-Rel-transformed lymphoid cells

Autor: Hodgson, J, Enrietto, P J
Zdroj: The Journal of Virology; March 1995, Vol. 69 Issue: 3 p1971-1979, 9p
Abstrakt: Constitutive and inducible kapp B binding activities associated with v-Rel and c-Rel in the cytosol of v-Rel-transformed cells have been identified. These activities were resolved by electrophoretic mobility shift assay and chromatographic techniques into a high-molecular-weight protein-DNA complex designated complex I containing v- and c-Rel and lower-molecular-weight complexes II, III and IV which contained only v-Rel and which were stimulated by nucleotides, low pH, and detergent. These experiments suggest that interaction of v-Rel and c-Rel decreases the DNA-binding activity of each.
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