| Autor: |
Rutberg, Lars, Armentrout, Richard W., Jonasson, Jon |
| Zdroj: |
The Journal of Virology; May 1972, Vol. 9 Issue: 5 p732-737, 6p |
| Abstrakt: |
SPO2 and f105 are temperate Bacillus subtilisbacteriophages which have been suggested to belong to a cluster of related bacteriophages. In the present work, we show that SPO2 does not complement any of the 11 essential genes known in f105 and that the phages do not recombine. Deoxyribonucleic acid (DNA)-DNA hybridization shows less than 10% homology between SPO2 and f105 DNA. DNA synthesis in f105 shows a greater dependence on host functions than does SPO2 DNA synthesis. Growth of f105 but not of SPO2 is inhibited by the uracil analogue 6-(p-hydroxyphenylazo)-uracil. Infection of a DNA polymerase-deficient strain of B. subtiliswith SPO2 leads to an increase in DNA polymerase activity in crude extracts, whereas no such increase is found after infection of this strain with f105. It is concluded that SPO2 and f105 are unrelated bacteriophages. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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