Correlation between in vivo and in vitro functional tests for activated macrophages

Autor: Rhodes, J M, Bennedsen, J, Larsen, S O, Riisgaard, S, Spärck, J V
Zdroj: Infection and Immunity; January 1979, Vol. 23 Issue: 1 p34-40, 7p
Abstrakt: Mice undergoing graft-versus-host reaction, skin grafting, and inoculation with tumor cells were tested for nonspecific resistance by intravenous challenge with Listeria monocytogenes. Peritoneal exudate macrophages from mice treated in a similar manner were tested in vitro for increased degradation of [1-14C]glucose, ability to degrade antigen/antibody complexes, ability to inhibit intracellular growth of listeria, and staining for beta-galactosidase. There was good correlation between in vivo resistance towards L. monocytogenes and in vitro inhibition of intracellular growth. There was also good correlation between increase in beta-galactosidase and in vivo resistance in mice undergoing a graft-versus-host-reaction.
Databáze: Supplemental Index