| Abstrakt: |
Certain dental plaques, removed from sites of gingival and periodontal pathology in mentally retarded, institutionalized individuals, when incubated in phosphate buffer with Achilles tendon collagen, gave rise to an increase in ninhydrin-positive material. These plaques, while showing great variability, released significantly more ninhydrin-positive material per milligram of plaque (wet weight) than did either the endogenous or heat-treated controls. Certain plaques could also break down soluble, tritiated, labeled collagen isolated from the calvaria of chicken embryos. Bacteroides melaninogenicusand Clostridia histolyticumwere found in plaques by either fluorescent antibody or cultural methods. C. histolyticum, when detected, accounted for about 0.01 to 0.1% of the bacteria in plaque. A conspicuous isolate from some plaques was a Bacillusspecies which rapidly liquefied gelatin. Cell-free supernatants of this organism were able to degrade about 50 to 70% of the soluble collagen when incubated at 36 C. C. histolyticumATCC 8034 caused an 80% degradation of the collagen under the same conditions of incubation. The Bacillusstrains were facultative, could ferment glucose, reduced nitrate to nitrite, and were catalase, indole, and urease negative. The limited taxonomic information for the isolates is compatible with the description given for Bacillus cereus. |
