| Autor: |
Thompson, Seona, Croft, Nirvana J., Sotiriou, Antonis, Piggins, Hugh D., Crosthwaite, Susan K. |
| Zdroj: |
Eukaryotic Cell; September 2008, Vol. 7 Issue: 9 p1573-1581, 9p |
| Abstrakt: |
ABSTRACTAppropriate responses of organisms to heat stress are essential for their survival. In eukaryotes, adaptation to high temperatures is mediated by heat shock transcription factors (HSFs). HSFs regulate the expression of heat shock proteins, which function as molecular chaperones assisting in protein folding and stability. In many model organisms a great deal is known about the products of hsfgenes. An important exception is the filamentous fungus and model eukaryote Neurospora crassa. Here we show that two Neurospora crassagenes whose protein products share similarity to known HSFs play different biological roles. We report that heat shock factor 1(hsf1) is an essential gene and that hsf2is required for asexual development. Conidiation may be blocked in the hsf2knockout (hsf2KO) strain because HSF2 is an integral element of the conidiation pathway or because it affects the availability of protein chaperones. We report that genes expressed during conidiation, for example fluffy, conidiation-10, and repressor of conidiation-1show wild-type levels of expression in a hsf2KOstrain. However, consistent with the lack of macroconidium development, levels of easare much reduced. Cultures of the hsf2KOstrain along with two other aconidial strains, the fluffyand aconidial-2strains, took longer than the wild type to recover from heat shock. Altered expression profiles of hsp90and a putative hsp90-associated protein in the hsf2KOstrain after exposure to heat shock may in part account for its reduced ability to cope with heat stress. |
| Databáze: |
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