| Autor: |
Marker, Stephen C., Gray, Ernest D. |
| Zdroj: |
Applied Microbiology; February 1972, Vol. 23 Issue: 2 p368-371, 4p |
| Abstrakt: |
A simple method for preparation and fractionation of the streptococcal nucleases by polyacrylamide gel electrophoresis is presented. The procedure is carried out with ammonium sulfate-precipitated supernatant fluids from cultures of beta-hemolytic streptococci grown to stationary phase. Electrophoresis on polyacrylamide gel and subsequent elution of the fractionated enzymes allows the preparation of sufficient homogeneous nuclease B for a large number of anti-nuclease B titrations. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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