| Autor: |
de Siqueira, Kátia Aparecida, Liotti, Rhavena Graziela, de Sousa, Janaina Rosa, Vendruscullo, Suzana Junges, de Souza, Gilza Barcelos, de Vasconcelos, Leonardo Gomes, Januário, Ana Helena, de Oliveira Mendes, Tiago Antônio, Soares, Marcos Antônio |
| Zdroj: |
3 Biotech; September 2021, Vol. 11 Issue: 9 |
| Abstrakt: |
Actinobacteria is a phylum composed of aerobic, Gram-positive, and filamentous bacteria with a broad spectrum of biological activity, including antioxidant, antitumor, and antibiotic. The crude extract of Streptomyces griseocarneusR132 was fractionated on a C18 silica column and the isolated compound was identified by 1H and 13C nuclear magnetic resonance as 3-(phenylprop-2-enoic acid), also known as trans-cinnamic acid. Antimicrobial activity against human pathogens was assayed in vitro (disk-diffusion qualitative test) and in vivo using Galleria mellonellalarvae (RT-qPCR). The methanol fractions 132-F30%, 132-F50%, 132-F70%, and 132-F100% inhibited the Escherichia coli(ATCC 25922) and Staphylococcus aureus(MRSA) growth in vitro the most effectively. Compared with the untreated control (60–80% of larvae death), the fractions and isolated trans-cinnamic acid increased the survival rate and modulated the immune system of G. mellonellalarvae infected with pathogenic microorganisms. The anti-infection effect of the S. griseocarneusR132 fermentation product led us to sequence its genome, which was assembled and annotated using the Rast and antiSMASH platforms.The assembled genome consisted of 227 scaffolds represented on a linear chromosome of 8.85 Mb and 71.3% of GC. We detected conserved domains typical of enzymes that produce molecules with biological activity, such as polyketides and non-ribosomal and ribosomal peptides, indicating a great potential for obtaining new antibiotics and molecules with biotechnological application. |
| Databáze: |
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