| Autor: |
Thambi, N C, Quan, F, Wolfgang, W J, Spiegel, A, Forte, M |
| Zdroj: |
Journal of Biological Chemistry; November 1989, Vol. 264 Issue: 31 p18552-18560, 9p |
| Abstrakt: |
The α subunits of heterotrimeric G proteins are responsible for the coupling of receptors for a wide variety of stimuli to a number of intracellular effector systems. In the nervous system of vertebrates, high levels of a specific class of G protein (Goα) are expressed. The α subunit of Goserves as a substrate for modification by pertussis toxin (PTX). In this report, we demonstrate that the Drosophilaheads contain high levels of a 40-kDa PTX substrate. Modification of this protein by PTX is modulated in a manner similar to that observed for vertebrate G proteins. The PTX substrate in Drosophilais also recognized specifically by antibodies raised against peptide sequences found specifically in vertebrate Goα. Vertebrate Goαprobes were used to identify a Drosophila cDNA coding for a potential PTX substrate with high sequence identity (82%) to vertebrate Goα. An additional cDNA coding for a related Goαhas also been isolated. The two cDNAs differ only in the 5′-untranslated and amino-terminal regions of the protein. This observation, in addition to Northern analysis, suggests that alternate splicing may generate a variety of Goα-like proteins in Drosophila. In situ hybridization of specific probes to tissue sections indicates that the mRNAs coding for Goα-like proteins in Drosophila are expressed primarily in neuronal cell bodies and, at lower levels, in the eyes. |
| Databáze: |
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