| Autor: |
Souza, Sandra C., de Vargas, Lina Moitoso, Yamamoto, Mia T., Lien, Ping, Franciosa, Mark D., Moss, Larry G., Greenberg, Andrew S. |
| Zdroj: |
Journal of Biological Chemistry; September 1998, Vol. 273 Issue: 38 p24665-24669, 5p |
| Abstrakt: |
Perilipins, a family of phosphoproteins, are specifically located at the surface of intracellular lipid (triacylglycerol) droplets, the site of lipolysis. Stimulation of lipolysis in 3T3-L1 adipocytes by tumor necrosis factor α (TNF-α) is associated with a decrease in total cellular expression of perilipin A and B, consistent with the hypothesis that a decrease in perilipin protein expression is required for TNF-α-induced lipolysis. Adenovirus-mediated overexpression of perilipin A or B maintains perilipin protein levels on the lipid droplet and blocks TNF-α-induced lipolysis. In contrast, overexpression of perilipin A or perilipin B does not inhibit isoproterenol-stimulated lipolysis and does not alter the isoproterenol-induced migration of perilipins from the lipid droplet. These results provide the first evidence of how perilipin functions and suggest that TNF-α regulates lipolysis, in part, by decreasing perilipin protein levels at the lipid droplet surface. |
| Databáze: |
Supplemental Index |
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