| Autor: |
Kuner, Pascal, Bohrmann, Bernd, Tjernberg, Lars O., Näslund, Jan, Huber, Gerda, Celenk, Suna, Grüninger-Leitch, Fiona, Richards, J.Grayson, Jakob-Rœtne, Roland, Kemp, John A., Nordstedt, Christer |
| Zdroj: |
Journal of Biological Chemistry; January 2000, Vol. 275 Issue: 3 p1673-1678, 6p |
| Abstrakt: |
The Alzheimer β-amyloid peptide (Aβ) and a fragment of the prion protein have the capacity of forming amyloid-like fibrils when incubated under physiological conditions in vitro. Here we show that a small amyloid ligand, RO-47-1816/001, enhances this process severalfold by binding to amyloid molecules and apparently promote formation of the peptide-to-peptide bonds that join the monomers of the amyloid fibrils. This effect could be antagonized by other ligands, including analogues of RO-47-1816/001, as well as the structurally unrelated ligand Congo red. Analogues of RO-47-1816/001 with low affinity for amyloid did not display any antagonistic effect. In conclusion, these data suggest that synthetic molecules, and possibly also small natural substances present in the brain, may act in a chaperone-like fashion, promoting Aβ polymerization and growth of amyloid fibrils in vitroand possibly also in vivo. Furthermore, we demonstrate that small organic molecules can be used to inhibit the action of amyloid-enhancing compounds. |
| Databáze: |
Supplemental Index |
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