| Autor: |
Ludikhuize, Marlies C., Meerlo, Maaike, Burgering, Boudewijn M.T., Rodríguez Colman, Maria J. |
| Zdroj: |
STAR Protocols; 20210101, Issue: Preprints |
| Abstrakt: |
Addressing bioenergetics is key to evaluate the impact of metabolism on the regulation of biological processes and its alteration in disease. Organoids are self-organizing structures derived from healthy and diseased tissue that closely recapitulate the tissue of origin. Bioenergetics is commonly analyzed by Seahorse-XF analysis. However, its application to organoid studies is technically challenging. Here we share our ‘in-house’ optimized protocols to examine organoid bioenergetics in response to drugs, gene knockdown or to characterize the metabolism of specific cell types. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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