| Autor: |
Raabová, Hedvika, Háková, Martina, Havlíková, Lucie Chocholoušová, Erben, Jakub, Chvojka, Jiří, Solich, Petr, Švec, František, Šatínský, Dalibor |
| Zdroj: |
Analytical Chemistry; May 2020, Vol. 92 Issue: 10 p6801-6805, 5p |
| Abstrakt: |
Poly-ε-caprolactone nanofibrous polymer has been used as an alternative to restricted access media for extraction of protein-containing biological samples and direct transfer in the chromatographic system. Three commercial cartridges differing in length and internal diameter have been manually packed with the composite material prepared from poly-ε-caprolactone nanofibers coated on poly-ε-caprolactone microfibrous scaffold and connected to the column-switching chromatographic system. Bovine milk and human serum (25 μL) spiked with a mixture of methyl-, ethyl-, propyl-, and butylparaben in a concentration range of 1–100 μg mL–1were online extracted using the cartridge-containing fibers. Then, 5 and 20% (v/v) aqueous methanol was applied as the washing mobile phase. While the ballast protein macromolecules were quantitatively eluted from the nano/microfibrous composite sorbent, the parabens were retained. After the mobile phase was switched to a stronger one, these compounds were then eluted from the extraction sorbent, directed in the analytical column, and finally separated. An extraction efficiency of 86–101% for all parabens achieved using the optimum-sized cartridge and a repeatability of the extraction procedure of 0.06–1.95% RSD were obtained. |
| Databáze: |
Supplemental Index |
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