| Autor: |
Vallejo, R L, Liu, H C, Cheng, H H, Witter, R L, Bacon, L D, Pharr, G T |
| Zdroj: |
Animal Genetics; October 1997, Vol. 28 Issue: 5 p331-337, 7p |
| Abstrakt: |
Marek’s disease (MD) is a lymphoproliferative disease caused by a member of the herpesvirus family, and the best understood genetic resistance to MD involves the chicken major histocompatibility complex (MHC) B‐complex. Preliminary observations have suggested that MHC‐like Rfp‐Ygenes might also influence the incidence of MD. This study describes the differentiation and definition of unique Rfp‐Ygenes in inbred lines 63and 72, lines that possess identical B‐complex genes, but that are resistant or susceptible to MD, respectively. To assess if Rfp‐Ygenes affect susceptibility to MD, 265 63× 72F2chickens were challenged with the JMstrain of MD virus at 1 week of age and were evaluated for MD lesions at up to 10 weeks of age. Genotyping of the F2chickens for Rfp‐Yhaplotypes was performed by restriction fragment length polymorphism analysis of genomic DNA using TaqI and a B‐FIVprobe. Analysis of variance and interval mapping procedures were used to determine association between the Rfp‐Yhaplotypes and the phenotypic MD values of the F2chickens. The cosegregation analysis of 265 F2chickens indicated that there was no association between Rfp‐Yhaplotypes and MD susceptibility. Furthermore, the fact that the Rfp‐Yhaplotypes fit the 1:2:1 segregation ratio and the Rfp‐Yallele frequencies did not differ significantly from 0·5 in the full population or in selected subpopulations (of either 40 MD‐resistant or 39 MD‐susceptible chickens) also indicated that Rfp‐Yhaplotypes do not significantly influence MD susceptibility. We conclude that Rfp‐Yhaplotypes do not play a major role in determining the genetic susceptibility to MD in 63× 72F2White Leghorn chickens. |
| Databáze: |
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