| Autor: |
Isiegas, C., Parro, V., Mellado, R. P. |
| Zdroj: |
Letters in Applied Microbiology; April 1999, Vol. 28 Issue: 4 p321-326, 6p |
| Abstrakt: |
The regulatory region and the region coding for the signal peptide of an extracellular agarase have been used to synthesize and secrete the heterologous Escherichia coliTEM β‐lactamase in Streptomyces lividans. The transcriptional regulation of the chimeric gene, and the secretion pattern of the chimeric gene product, coincided with those of the agarase gene. The negative glucose effect on the secretion of the protein was reverted when the recombinant bacterium was grown in the chemostat under phosphate limiting conditions. |
| Databáze: |
Supplemental Index |
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