| Abstrakt: |
ZhaoX., WangX., ZhouJ., ChenB., ZengA., BaoQ. andWangH. 2013. Proteomic analysis and qRT-PCR verification of Arthrospira platensisstrain YZ under dark stress. Phycologia52: 538–549. DOI: 10.2216/13-167.1Arthrospira platensisis an ideal organism for a proteome study under conditions of environmental stress. Herein, we report the differential expression of proteins in A. platensisstrain YZ responding to 48 h of continuous dark or light conditions by two-dimensional gel electrophoresis and peptide mass fingerprinting techniques. There were 102 proteins retrieved with significant differential expression between light and dark incubation, among which 96 protein spots were found with high homology to Arthrospiragenus (67 proteins in A. platensisstrain Paraca and 29 in A. maximastrain CS-328). The other six proteins belonged to other microorganisms. Using a clusters of orthologous groups database, we classified the 102 differentially expressed positive proteins into 19 types based on their function and localized them in their respective KEGG metabolic pathways. The proteins were involved in 47 metabolic pathways, such as photosynthesis, glycolysis/gluconeogenesis pathway, oxidative phosphorylation, and amino acid and fatty acid synthesis. These results implied that the proteins performed predictable roles in rendering A. platensisstrain YZ resistant against dark stress. Additionally, we determined the transcriptional level of 26 genes in response to dark stress in vivoby quantitative reverse-transcription polymerase chain reaction, and we found that the expression of six differential proteins, representing 23.08% of the total target genes, was consistent between transcriptional and translational levels. The remaining 20 genes showed inconsistent protein expression compared with the transcriptional level, which accounted for 76.92% of the total target genes. |
