| Autor: |
KATSUMORI, TAKASHI, YAMAGUCHI, YASUO, MORI, KATSUTAKA, MAKINO, YASUHIRO, MISUMI, MIKIO, TAKATA, NOBORU, GOTO, MATAROU, KIKUCHI, NOBUYUKI, OGAWA, MICHIO |
| Zdroj: |
Transplantation; September 1992, Vol. 54 Issue: 3 p531-536, 6p |
| Abstrakt: |
A single intravenous injection of 1 ml freshly heparinized donor blood seven days before transplantation significantly prolonged the survival of subsequent donorspecific hepatic allografts in the fully allogeneic ACI(RT1a-to-LEW(RT11) rat combination. The time course of cell-mediated lympholysis was studied in this animal model. The activity of CML in lymphocytes infiltrating into the hepatic allograft (CML-G) and in the spleen (CML-S) was determined by measuring lysis of donor Con A blast cervical lymph node cells. Preoperative DST resulted in an increased activity of CML-S with a peak (31.6, E/T = 150) on day 7. This increased CML-S activity after DST rapidly declined during the first days following hepatic transplantation. The activities of both CML-S and CML-G then increased after transplantation and reached peaks on days 15 (48, E/T = 150) and 20 (2.57, E/T = 75), respectively. These were much higher than the peak values of CML-S (11.2, E/T = 150) on day 7 and CML-G (19.5, E/T = 75) on day 6 in untreated controls and were followed by a subsequent gradual decrease in those activities to pre-operative levels by day 113 posttransplant. Phenotypic analysis of lymphocytes infiltrating grafts in DST-treated hosts demonstrated that the CD4/CD8 ratio remained relatively constant (<1.0). While the ratio in control grafts increased and reached a peak (2.17) on day 9. Histological examination revealed that mononuclear cell infiltration of grafts reached a peak on day 9 in both DST-treated hosts and controls. This mononuclear cell accumulation gradually subsided in DST-enhanced grafts. The mitotic index of graft hepatocytes reached a peak on day 15 in DST-treated hosts and on day 7 in control. |
| Databáze: |
Supplemental Index |
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