| Autor: |
TONG, JENNIFER Y., HSIA, SHYUAN, PARRIS, GEORGE L., NGHIEM, DAI D., COTTINGTON, ERIC M., RUDERT, WILLIAM A., TRUCCO, MASSIMO |
| Zdroj: |
Transplantation; February 1993, Vol. 55 Issue: 2 p390-394, 5p |
| Abstrakt: |
Renal donor-recipient HLA DQB1 typing at the DNA level provides a new avenue to study graft survival (GS) and compatibility. HLA DQB1 genotypes of 63 renal donor-recipient pairs were typed simultaneously by assessing patterns from electrophoresed restriction fragments (PCR-RFLP) of amplified DNA (ADNA) and hybridization patterns of sequence specific oligonucleotides (PCR-SSO) of ADNA. Thus the clinical applicability of these two protocols for HLA DQB1 typing was assessed in addition to the compatibility study. Typing results of these two protocols gave overall agreeable results. Sixty-seven per cent of 150 alleles from 75 heterozygotes typed by both protocols had identical allelic type assignments. Serotyping shared more concordant results to PCR-RFLP determined types than to PCR-SSO determined types. The PCR-RFLP protocol can be easily implemented for clinical DNA typing because of its clarity in assigning allelic types and the possible handling of a small number of typing samples, even a single sample, in a single run. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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