| Autor: |
Pinkert, C. A., Kooyman, D. L., Baumgartner, A., Keisler, D. H. |
| Zdroj: |
Reproduction; September 1989, Vol. 87 Issue: 1 p63-66, 4p |
| Abstrakt: |
Summary.Ten prepubertal and 8 mature gilts were superovulated with PMSG and hCG, and inseminated with fresh boar semen. Zygotes were surgically recovered from oviducts 54–60 h after hCG. One and 2-cell zygotes were randomly allotted to Medium PL (modified BMOC-3 supplemented with 0·1 mm-EDTA and 1·5% BSA) or Medium G (Medium PL without pyruvate or lactate). Eggs were washed twice in medium, and placed in microdrops of medium overlaid with silicon oil for culture in an humidified 5% CO2, 5% O2, 90% N2environment, then observed daily for 6 days. Development of eggs was dependent (P< 0·001) on the interactive effects of age of gilt (prepubertal versusmature) and medium type (PL versusG) used in culture. A greater proportion of eggs cultured in Medium G developed further than did eggs in Medium PL (P< 0·001). Additionally, a greater proportion of eggs from mature gilts developed further than did eggs from prepubertal gilts (P< 0·02). We suggest that these results provide evidence that zygotes resulting from superovulation regimens of prepubertal gilts do not possess the same capacity for in-vitro development as do zygotes from pubertal gilts.Keywords:zygote; blastocyst; prepubertal; pigs |
| Databáze: |
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