| Autor: |
Steidley, Kenneth E., Thompson, Stevan H., McQuade, Michael J., Strong, Scott L., Scheidt, Michael J., Van Dyke, Thomas E. |
| Zdroj: |
Journal of Periodontology; September 1992, Vol. 63 Issue: 9 p753-756, 4p |
| Abstrakt: |
Previous reports describe a characteristic, rapidly progressive, Periodontitis that is unique to patients who are seropositive for HIV antibody (Western blot +). The purpose of this study was to compare the T4 and T8 lymphocyte subpopulations in the peripheral blood and periodontal lesions of these HIV patients with those of healthy controls. T‐cell subsets in peripheral blood were quantified by flow cytometry. The values from this analysis were used to calculate the peripheral T4:T8 lymphocyte ratio for each patient. Gingival tissue (papilla) was obtained from 8 HIV + patients and from 6 healthy HIV− control patients during routine gingival surgery. The T‐cell subpopulations in the gingival tissue were determined using serial cryostat sections that were labeled with monoclonal antibodies for T4 and T8 cells and developed using an avidin‐biotin‐peroxidase system. Six sections were taken from each of the 14 tissue specimens (one per patient). The sections were examined at 450 × and the mean number of T4 and T8 cells calculated for each section. These mean values were then used to determine the T4:T8 lymphocyte ratio for each tissue specimen. The peripheral blood analysis revealed a mean serum T4:T8 ratio of (2.07 ± 0.455) for the controls and (0.58 ± 0.26) for the HIV patients. The significantly lower T4:T8 ratio in HIV patients is consistent with their diagnosis. Although the results indicated that the mean T4:T8 lymphocyte ratio in the gingiva of controls was highly variable (2.70 ± 1.344), the gingiva of HIV patients consistently exhibited a complete absence of T‐cells. The lack of locally present immune effector and regulatory cells in HIV seropositive patients with periodontal disease could in part explain the characteristic and rapidly progressive nature of their periodontal disease. J Periodontol 1992; 63:753–756. |
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