| Autor: |
Potrich, Michele, Silva, Rita T.L. da, Maia, Fabiana M.C., Lozano, Everton R., Rossi, Robson M., Colombo, Fernanda C., Tedesco, Flavia G., Gouvea, Alfredo de |
| Zdroj: |
Revista Brasileira de Entomologia; January-March 2018, Vol. 62 Issue: 1 p23-28, 6p |
| Abstrakt: |
This study aimed to evaluate the effect of commercially used entomopathogens on Africanized Apis melliferaL. (Hymenoptera: Apidae). Four bioassays were performed: 1) pulverized entomopathogens on A. mellifera; 2) entomopathogens sprayed on a smooth surface; 3) entomopathogens sprayed on soy leaves; and 4) entomopathogens mixed with candy paste (sugar syrup). Five treatments were prepared: sterile distilled water (control), distilled water sterilized with Tween®80 (0.01%), and the commercial entomopathogens Metarhizium anisopliaeE9 (1.0×109conidiamL−1), Beauveria bassianaPL63 (1.0×108conidia mL−1) and Bacillus thuringiensisvar. kurstaki HD-1 (3.0×108sporesmL−1). Each treatment consisted of five repetitions, with 20 workers per repetition, which were stored in a plastic box and, later, in a biological oxygen demand (B.O.D.) incubator (27±2°C, RH of 60%±10%, 12-h photophase). The mortality of the workers was evaluated from 1h to 240h, and the data were analyzed using Bayesian inference. The workers killed by the ingestion of candy paste contaminated with the pathogens (products) were randomly separated and selected for the removal of the midgut. Each midgut was fixed in Bouin's solution and prepared for histology. B. bassianawas verified to reduce the survival of A. melliferaworkers in all bioassays. Moreover, M. anisopliaereduced the survival of A. melliferaworkers directly sprayed, on a smooth surface and mixed with candy. B. thuringiensisreduced A. melliferasurvival on a smooth surface and mixed with candy paste. However, its effects were lower than that observed by B. bassiana.The treatments with the biological products did not induce morphometric alterations in the midgut of A. mellifera. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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