| Autor: |
Yang, Jingwen, Xu, Yuchao, Xu, Ke, Ping, Hongling, Shi, Huilai, Lü, Zhenming, Wu, Changwen, Wang, Tianming |
| Zdroj: |
Chinese Journal of Oceanology and Limnology; 20240101, Issue: Preprints p1-13, 13p |
| Abstrakt: |
Neuropeptide Y (NPY) has a pivotal role in the regulation of many physiological processes. In this study, the gene encoding a NPY receptor-like from the common Chinese cuttlefish Sepiella japonica(SjNPYR-like) was identified and characterized. The full-length SjNPYR-likecDNA was cloned containing a 492-bp of 5′ untranslated region (UTR), 1 182 bp open reading frame (ORF) encoding a protein of 393 amino acid residues, and 228 bp of 3′ UTR. The putative protein was predicted to have a molecular weight of 45.54 kDa and an isoelectric point (pI) of 8.13. By informatic analyses, SjNPYR-like was identified as belonging to the class A G protein coupled receptor (GPCR) family (the rhodopsin-type). The amino acid sequence contained 12 potential phosphorylation sites and five predicted N-linked glycosylation sites. Multiple sequence alignment and 3D structure modeling were conducted to clarify SjNPYR bioinformatics characteristics. Phylogenetic analysis identifies it as an NPYR with identity of 33% to Lymnaea stagnalisNPFR. Transmembrane properties of SjNPYR-like were demonstrated in vitro using HEK293 cells and the pEGFP-N1 plasmid. Relative quantification of SjNPYR-like mRNA level confirmed a high level expression and broad distribution of SjNPYR - likein various tissues of female S. japonica. In addition, the transcriptional profile of SjNPYR - likein the brain, liver, and ovary during gonadal development was analyzed. The results provide basic understanding on the molecular characteristics of SjNPYR-like and its potentially physical functions. |
| Databáze: |
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