| Autor: |
Ma, A., Fisher, P., Dildrop, R., Oltz, E., Rathbun, G., Achacoso, P., Stall, A., Alt, F.W. |
| Zdroj: |
EMBO Journal; July 1992, Vol. 11 Issue: 7 p2727-2734, 8p |
| Abstrakt: |
Transgenic mice carrying either the c‐myc or N‐myc oncogene deregulated by the immunoglobulin heavy chain enhancer element (E mu) develop both pre‐B and B cell lymphomas (E mu‐c‐myc and E mu‐N‐myc lymphomas). We report here that B cell lines derived from these tumors, as well as a line derived from v‐myc retroviral transformation, simultaneously express surface immunoglobulin (a hallmark of mature B cells) as well as a common subset of genes normally restricted to the pre‐B stage of development‐including the recombinase activating genes RAG‐1 and RAG‐2. Continued RAG‐1 and RAG‐2 expression in these lines is associated with VDJ recombinase activity detected with a VDJ recombination substrate. Cross‐linking of the surface immunoglobulin on these lines with an anti‐mu antibody leads to rapid, specific and reversible down‐regulation of RAG‐1 and RAG‐2 gene expression. We also find that a small but significant percentage of normal surface immunoglobulin bearing bone marrow B cells express the RAG‐1 gene. These findings are discussed in the context of their possible implications for the control of specific gene expression during the pre‐B to B cell transition. |
| Databáze: |
Supplemental Index |
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