| Autor: |
To, Ho, Teshima, Kaho, Nagai, Shinya, Zielinski, Gustavo C., Koyama, Tomohiro, Lee, Jina, Bessone, Fernando A., Nagano, Tetsuji, Oshima, Atsushi, Tsutsumi, Nobuyuki |
| Zdroj: |
Revista Argentina de Microbiologia; January-March 2018, Vol. 50 Issue: 1 p12-22, 11p |
| Abstrakt: |
The objectives of this study were to determine the serovar of a collection of Actinobacillus pleuropneumoniaestrains within the 3-6-8-15 cross-reacting group and to analyze their phenotypic and genetic properties. Based on the serological tests, forty-seven field strains of Actinobacillus pleuropneumoniaeisolated from lungs with pleuropneumonia lesions in Japan and Argentina were found to be serovars belonging to the 3-6-8-15 cross-reacting group. By using a capsule loci-based PCR, twenty-nine (96.7%) and one (3.3%) from Japan were identified as serovars 15 and 8, respectively, whereas seventeen (100%) from Argentina were identified as serovar 8. The findings suggested that serovars 8 and 15 were prevalent within the 3-6-8-15 cross-reacting group, in Argentina and Japan, respectively. Phenotypic analyses revealed that the protein patterns observed on SDS–PAGE and the lipopolysaccharide antigen detected by immunoblotting of the reference and field strains of serovars 8 and 15 were similar to each other. Genetic (16S rDNA, apxIIA, apxIIIA, cps, cpxgenes, apxand omlApatterns) analyses revealed that the apxIIAand apxIIIAgenes of the field strains of serovars 8 and 15 were similar to those of the reference strains of serovars 3, 4, 6, 8 and 15. The results obtained in the present study may be useful for the development of more effective vaccines against disease caused by A. pleuropneumoniaeby including the homologous antigens to the most prevalent serovars in specific geographical areas. |
| Databáze: |
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